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dc.contributor.authorBrinkmann, Bregje W
dc.contributor.authorBeijk, Wouter F
dc.contributor.authorVlieg, Redmar C
dc.contributor.authorvan Noort, S John T
dc.contributor.authorMejia, Jorge
dc.contributor.authorColaux, Julien L
dc.contributor.authorLucas, Stéphane
dc.contributor.authorLamers, Gerda
dc.contributor.authorPeijnenburg, Willie J G M
dc.contributor.authorVijver, Martina G
dc.date.accessioned2021-02-07T14:58:06Z
dc.date.available2021-02-07T14:58:06Z
dc.date.issued2021-01-24
dc.identifier.pmid33535134
dc.identifier.doi10.1016/j.aquatox.2021.105744
dc.identifier.urihttp://hdl.handle.net/10029/624669
dc.description.abstractTeleost fish embryos are protected by two acellular membranes against particulate pollutants that are present in the water column. These membranes provide an effective barrier preventing particle uptake. In this study, we tested the hypothesis that the adsorption of antimicrobial titanium dioxide nanoparticles onto zebrafish eggs nevertheless harms the developing embryo by disturbing early microbial colonization. Zebrafish eggs were exposed during their first day of development to 2, 5 and 10 mg TiO2 L-1 (NM-105). Additionally, eggs were exposed to gold nanorods to assess the effectiveness of the eggs' membranes in preventing particle uptake, localizing these particles by way of two-photon microscopy. This confirmed that particles accumulate onto zebrafish eggs, without any detectable amounts of particles crossing the protective membranes. By way of particle-induced X-ray emission analysis, we inferred that the titanium dioxide particles could cover 25-45 % of the zebrafish egg surface, where the concentrations of sorbed titanium correlated positively with concentrations of potassium and correlated negatively with concentrations of silicon. A combination of imaging and culture-based microbial identification techniques revealed that the adsorbed particles exerted antimicrobial effects, but resulted in an overall increase of microbial abundance, without any change in heterotrophic microbial activity, as inferred based on carbon substrate utilization. This effect persisted upon hatching, since larvae from particle-exposed eggs still comprised higher microbial abundance than larvae that hatched from control eggs. Notably, pathogenic aeromonads tolerated the antimicrobial properties of the nanoparticles. Overall, our results show that the adsorption of suspended antimicrobial nanoparticles on aquatic eggs can have cascading effects across different life stages of oviparous animals. Our study furthermore suggests that aggregation dynamics may occur that could facilitate the dispersal of pathogenic bacteria through aquatic ecosystems.en_US
dc.language.isoenen_US
dc.rightsCopyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
dc.subjectCascading effectsen_US
dc.subjectEcoPlateen_US
dc.subjectHost-microbiota interactionsen_US
dc.subjectParticle-induced X-ray emissionen_US
dc.subjectTwo-photon multifocal microscopyen_US
dc.titleAdsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota.en_US
dc.typeArticleen_US
dc.identifier.eissn1879-1514
dc.identifier.journalAquat Toxicol 2021; advance online publication (ahead of print)en_US
dc.source.journaltitleAquatic toxicology (Amsterdam, Netherlands)
dc.source.volume232
dc.source.beginpage105744
dc.source.endpage
dc.source.countryNetherlands


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