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Cell lines and SalmonellaJonge R de; Hendriks H; Garssen J; Universteit Utrecht, afdeling Pathologie; MGB; LPI (Rijksinstituut voor Volksgezondheid en Milieu RIVM, 2001-08-09)In human gastrointestinal disease caused by Salmonella, transepithelial migration of neutrophils follows the attachment of bacteria to epithelial tissue. This migration of neutrophils is stimulated by the release of chemokines, including interleukin-8 (Il -8), from the epithelial cells. We have developed an in vitro model system (human epithelial monolayers, among which Caco-2 cells grown on microtiter multiwell plates) for studying host-pathogen interactions. After infection with different pathogens we measured Il-8 production during time. Results showed that Il-8 release was time related and varied with the pathogen. Salmonella enteritidis (Se) did induce the highest response. Subsequently, three doses of this Se strain were used and the Il-8 response was measured at different time points. Caco-2 cells remained intact over a period of 24h, the production of Il-8 increased in time and was found to be Se dose-dependent. Other tested epithelial monolayers, such as HT29 colon cancer cells, gave similar results.